Qiagen tissueruptor user manual

See disruption and homogenization using the tissueruptor, page 17, or disruption and homogenization using the tissuelyser system, page 17. The tissueruptor is a handheld rotorstator homogenizer that provides rapid, efficient, and flexible disruption of human, animal, and plant samples for a wide range of downstream applications. Manual vacuum purification of total rna mirna poly a plus mrna dna or protein. Refer to the tissueruptor user manual or the tissuelyser handbook for operating instructions. The purchaser and user of the kit agree not to take or permit anyone else to take any. Use of the tissueruptor or the tissuelyser, for rapid and convenient. Fragments ranging from 100 bp to 10 kb are purified from primers, nucleotides, polymerases, and salts using qiaquick spin columns in a microcentrifuge. Pcr purification qiaquick kit protocol this protocol is designed to purify single or doublestranded dna fragments from pcr. Qiaquick gel extraction kit protocol using a microcentrifuge. Qiagen multiplex pcr kit, x 50l rxns, genomic dna and cdna sample, pcr amplification reaction, 5 3 exonuclease enzyme activity, easytouse and costeffective, for highly specific and sensitive multiplex pcr without optimization requirements, includes 2x qiagen. The blade of the probe rotates at a very high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Qiagen dna and rna purifications qiagen qiaamp circulating.

Rapid, effective disruption of a range of sample types. Disruption and homogenization using the tissueruptor or other rotorstator. Rapid, effective disruption of a range of sample types disposable probes help to minimize crosscontamination time savings through use of disposable probes visual monitoring of disruption using transparent probes seamless integration with qiagen sample technologies the tissueruptor ii is a handheld. This model provides rapid and effect disrupting, it is compatible with disposable probes to prevent cross contamination, it has a speed range from 5000 to 35000 rpm, and has a typical run time between 15 and 120 seconds. For low throughput disruption of biological samples using disposable probes. Freecirculating dna,rna and mirna,viral dna,viral rna. Adapter sets for flexible sample throughput 2 x 24, 2 x 96. Each sample was vortexed for 30 seconds, centrifuged at 300 g for 10 minutes at 4c, and the supernatant was discarded.

L of lysis buffer rlt plus qiagen, hilden, germany cells were counted to establish the amount of rlt plus buffer and homogenized for 30 seconds with a tissueruptor qiagen at medium velocity. Sunlight, ovens, or environments with wide temperature. Operating manual sigma 415c, page 3 of 95 0306 dear customer, congratulations for purchasing a sigma laboratory centrifuge. Sequential automation of rna and dna preps on the same. Shearing performance is independent of the source, concentration, temperature, or salt content of a dna sample. All instructions and protocols in this handbook apply to both the tissuelyser ii and the. The tissueruptor ii is an integral part of qiagen s complete solution for sample management from sample collection to purification and analysis of dna, rna and protein.

Tissueruptor and tissuelyser systems are integral parts of qiagens. Can anyone suggest a protocol for rna extraction from. Automated purification with the ez1 dna tissue kit requires use of the ez1 advanced xl and the ez1 advanced xl dna tissue card, or the ez1 advanced and the ez1. Prior to isolation, carefully remove the tissue from the storage reagent using forceps and dry any excessive liquid.

The mirneasy mini kit enables lowthroughput rna purification using spin columns. Qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. Under these conditions, qiaquick spin kits can be stored for up to 12 months without showing any reduction in performance and quality. Qiagen clc genomics workbench software is used by hundreds of microbiology and virology labs around the world for basic research and infectious disease epidemiology. Sample to insight agenda 3 gdna introduction stabilization of samples sample disruption for extraction of genomic dna gdna isolation technologies handling and storing gdna.

Disposable probes help to eliminate crosscontamination. The instructions and safety information in the user manual must be followed to ensure safe operation of the ii and to tissueruptor. Print bookmark share more for low throughput disruption of biological samples using disposable probes. Ensure that you are familiar with operating the tissueruptor or the tissuelyser. For highly specific and sensitive multiplex pcr without optimization requirements. Rneasy lipid tissue handbook 022009 9 equipment and reagents to be supplied by user when working with chemicals, always wear a suitable lab coat, disposable gloves. Qiagen, biosprint, dneasy, magattract, rneasy, tissueruptor qiagen group. For cleanup of other enzymatic reactions, follow the protocol as described for pcr samples or use the minelute reaction cleanup kit. Qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. Tissueruptor disposable probes 25, 990890 from qiagen. Tissueruptor is an integral part of qiagens complete solution for tissue management. Your balance was designed and manufactured to give you years of service. Forpurificationoftotalrna,includingmirna,fromserumorplasmasamples,werec. Tissueruptor disposable probes 25, 990890 from qiagen labsave.

Vortex adapters pathogen lysis tubes power bead tubes. Tissueruptor, qiagen can be used for rapid and efficient tissue homogenization. Tissuelyser lt handbook qiagen gel electrophoresis. Qiagen kit handbooks and user manuals are available at. A wide spectrum of programming options and an electronic operation control allow a troublefree use of the centrifuge. Sample to insight tutorial otu clustering step by step september 2, 2019 qiagen aarhus silkeborgvej 2 prismet 8000 aarhus c denmark telephone. Limited license agreement for dneasy plant kits use of this product signifies the agreement of any purchaser or user of the product to the following terms.

Qiaquick spin kits should be stored dry at room temperature 1525c. For uptodate licensing information and productspecific disclaimers for qiagen products, see the respective qiagen kit handbook or user manual. Tissuelyser lt, or tissuelyser ii by referring to the respective user manual and handbook. Seamless integration with qiagen sample technologies rapid, effective disruption the tissueruptor is a portable device to which a tissueruptor disposable probe is attached. Congratulations and thank you for selecting a vwr a series balance, we appreciate your business.

Qiagen qiaamp dna ffpe tissue kit, 50 preps, 20 to 100l elution volume, up to 8 sections sample, formalin fixed paraffin embedded tissue sample, silica technology, genomic dna, mitochondrial dna purification, spin column format, ideal for realtime pcr, str analysis, lmdpcr. For purification of genomic dna from formalinfixed paraffinembedded tissues. Used qiagen tissueruptor electric corded hand held tissue. For longer storage, qiaquick spin kits can also be stored at 28c, but in this case the buffers should be redissolved before use.

Rna integrity and quality standardize rna quality control. You have selected a device which combines many advantages. Up to 400 mg agarose can be processed per spin column. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. Sequential automation of rna and dna preps on the same qiacube instrument 042017 3 high yields of highly pure plasmid dna as shown in figure 4, a yield of 9. Samples are simultaneously disrupted and homogenized by a rotating blade rotorstator homogenization. Dneasy blood and tissue kit 50 from qiagen sample to. Mott center 275 east hancock detroit, michigan 48201 fax. Mo bio kit manuals are available at or can be requested from mo bio technical services.

Rapid, effective disruption of a range of sample types disposable probes help to minimize crosscontamination time savings through use of disposable probes visual monitoring of disruption using transparent probes seamless integration with qiagen sample technologies the tissueruptor. Forhighthroughputpurificationina96wellformat,themirneasy96kitisavailable. We recommend all users of qiagen products to adhere to the. Disposable probes help to minimize crosscontamination time savings through use of disposable probes visual monitoring of disruption using transparent probes seamless integration with qiagen sample technologies. Qiagen multiplex pcr kit, 206145 from qiagen labsave. Supplier part number mfrr name item description model number uom published price 9018935 qiagen adapter, reagent tube sbs plate eqsv0isac0 each 528 9018938 qiagen adapter, light cycler 480 384well plate eqsv0isac0 each 406 9018942 qiagen adapter, 2xring of 12 cobas amplicore eqsv0isac0 each 509 9018953 qiagen reagent block, 16x0. Qiagen, sample to insight, qiacube, qiagility, qiasymphony, qiaxcel, qiaxpert, ez1, pyromark, pyrosequencing, rotorgene, tissueruptor qiagen group. Preanalytix products are manufactured for preanalytix by qiagen or bd and are distributed. Purification of total dna from fresh plant tissue dneasy 96 protocol. Fans, heatair duct, doorways or high traffic areas.

Qiaamp dna ffpe tissue kit 50, 56404 from qiagen labsave. Get access to some of qiagens cuttingedge technologies that will help you achieve the success that you desire. This kit can also be used for dna cleanup from enzymatic reactions see page 8. For lowthroughput sample disruption for molecular analysis.

Qiagen clc genomics has also been critical for many labs on the front lines of the covid19 pandemic involved in sequencing the sarscov2 virus. Ensure that you are familiar with operating the tissueruptor ii. Apr 28, 2017 qiagen kit handbooks and user manuals are available at. Equiequipment and reagents to be supplied by userpment and reagents to be supplied by userpment and reagents to be supplied by user 1. Quickstartprotocol qiazol lysis reagent january 2011.

Dneasy plant handbook the steve clough lab mafiadoc. This model provides rapid and effect disrupting, it is compatible with disposable probes to prevent cross contamination, it has a speed range from 5000 to 35000 rpm. Tissues stored in rna stabilization reagents such as rnalater are compatible with this isolation procedure. Used qiagen tissueruptor electric corded hand held tissue homogenizer in excellent working condition. This protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8.